Smart Taq Red Thermostable DNA Polymerase
| Cat No | Y2-104-005 | Y2-104-025 |
| Qty | 500 U | 2500 U |
| Source | Recombinant E.coli strain | |
| Concentration | 1 U/µl | |
| Storage | - 20 oC | |
Enzyme properties
- Smart Taq Red has a special formulation with added inert red dye. This makes it very suitable for standard applications.
- Strong red color of the enzyme allows user to check the polymerase addition and verify adequate mixing.
- Reaction products are ready for direct gel loading and the dye serves as marker for electrophoresis progress monitoring.
- Purified from an recombinant E.coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.
- Taq DNA polymerase has 5’-> 3’ DNA synthesis activity.
- Activity and stability tested at 20, 30 and 40 cycles of PCR reactions at 95°C.
- Tested for the absence of human DNA contamination by PCR with Alu-specific primers.
One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C. Storage Buffer
20 mM Tris-HCl (pH 8.0), 1mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Smart Taq Red Thermostable DNA Polymerase is supplied with 3 vials of working solution: 10X PCR Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonidet P40
10X PCR Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% Tween 20
25 mM MgCl2 Solution
